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Multiphoton fluorescence image of HeLa cells with cytoskeletal microtubules (magenta) and DNA (cyan)

Multiphoton fluorescence image of HeLa cells with cytoskeletal microtubules (magenta) and DNA (cyan). Nikon RTS2000MP custom laser scanning microscope.

Source: National Center for Microscopy and Imaging Research (NCMIR) (source)

Credit Line: National Center for Microscopy and Imaging Research


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Image of the Week – June 8, 2020

CIL:12375 -http://cellimagelibrary.org/images/12375

Description: Movie showing the dynamics of kinetchore microtubules during meiosis II in primary spermatocytes of the crane-fly Nephrotoma suturalis that were experimentally flattened. Time-lapse polarization microscopy using a Nikon Microphot SA, equipped for liquid crystal polarized light microscopy (LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 15 sec using a QImaging Retigo EXi CCD camera. Raw images were processed using 5-frame algorithm (Shribak and Oldenbourg, 2003). The time series used for the movie is included in this grouped set.

Authors: James R. LaFountain and Rudolf Oldenbourg

Licensing: Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible.

#biology    #science    #microtubules    
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