Can anyone help me understand it? I am not able to understand why +/- signs are written above the gel profile? And can anyone help me with the hint to the solution?
Authored by Kenny Walter, Digital Reporter, R&D Magazine
A new lightweight, high-powered wheelchair that is powered by high-pressurized air instead of batteries or electronics is offering users new recreational opportunities.
Researchers from the University of Pittsburgh have created the PneuChair, a pneumatic wheelchair that forgoes the traditional heavy battery energy source for high-pressured air. It runs on 4,500 psi. This make it both significantly lighter than other power chairs and also waterproof, enabling users to take the PneuChair to lakes, beaches and water parks.
Authored by Ryan Bushey, Digital Editor, Drug Discovery & Development
A new biotech startup has launched with a unique strategy for creating specialized cell and gene therapies.
Obsidian Therapeutics completed an estimated $49.5 million financing round as it seeks to advance its lead programs toward clinical development and continue building its proprietary technology platform.
This reminds me of that biotechnology that’s being developed that can be injected straight into a person’s mind, but instead of using it for like, you know, education, pleasure, medicine, etc., they’re planning on using it to torture convicts, making them believe they’ve served a 1000 year sentence.
Made these Biochemistry & Metabolism notes from last semester for my final exam :) It was my first time taking a 5 credit hour subject. Honestly, there were tons of stuffs + terms to memorize, especially the metabolic pathways. The nucleotide biosynthesis topic was really tough for me b/c I’ve never learn it before + I also had to memorize the diseases + syndromes along with how they affect the associated pathways. Well, I’m just glad that I got a GPA 4.0 for my Biochemistry & Metabolism! Hard work does pay off :)
Here are some pictures that I’ve taken from my past laboratory experiments during the whole foundation year + my first year of BSc (Hons) Biotechnology in university! Had some pictures that are from failed / unsuccessful experiments (ㆀ˘･з･˘)
Much of my research deals with the ways in which bodies are disciplined and how they go about resisting that discipline. In this piece, adapted from one of the answers to my PhD preliminary exams written and defended two months ago, I “name the disciplinary strategies that are used to control bodies and discuss the ways that bodies resist those strategies.” Additionally, I address how strategies of embodied control and resistance have changed over time, and how identifying and existing as a cyborg and/or an artificial intelligence can be understood as a strategy of control, resistance, or both.
In Jan Golinski’s Making Natural Knowledge, he spends some time discussing the different understandings of the word “discipline” and the role their transformations have played in the definition and transmission of knowledge as both artifacts and culture. In particular, he uses the space in section three of chapter two to discuss the role Foucault has played in historical understandings of knowledge, categorization, and disciplinarity. Using Foucault’s work in Discipline and Punish, we can draw an explicit connection between the various meanings “discipline” and ways that bodies are individually, culturally, and socially conditioned to fit particular modes of behavior, and the specific ways marginalized peoples are disciplined, relating to their various embodiments.
This will demonstrate how modes of observation and surveillance lead to certain types of embodiments being deemed “illegal” or otherwise unacceptable and thus further believed to be in need of methodologies of entrainment, correction, or reform in the form of psychological and physical torture, carceral punishment, and other means of institutionalization.
Feeding the world (and saving nature) in this populous century, Jane Langdale began, depends entirely on agricultural efficiency — the ability to turn a given amount of land and sunlight into ever more food. And that depends on three forms of efficiency in each crop plant: 1) interception efficiency (collecting sunlight); 2) conversion efficiency (turning sunlight into sugars and starch); and 3) partitioning efficiency (maximizing the edible part). Of these, after centuries of plant breeding, only conversion efficiency is far short of the theoretical maximum. Most photosynthesis (called “C3“) is low-grade, poisoning its own process by reacting with oxygen instead of carbon dioxide when environmental conditions are hot and dry.
Throughout history, humans have used plants in the treatment of disease. This includes more traditional methods involving direct consumption with minimal preparation involved and the extraction of compounds for use in modern pharmaceuticals. One of the more recent methods of using plants in medicine involves the synthesis and application of plantibodies and plant produced antigens. These are recombinant antibodies and antigens respectively, which have been produced by a genetically modified plant (1, 2).
Antibodies are a diverse set of proteins which serve the purpose of aiding the body in eliminating foreign pathogens. They are secreted by effector B lymphocytes which are a type of white blood cell that circulate throughout the body. An antigen is a molecule or a component of a molecule, such as a protein or carbohydrate, which can stimulate an immune response. The human body is capable of producing around 1012 different types of antibodies, each of which can bind to a specific antigen or a small group of related motifs (3). When an antibody encounters the antigen of a foreign pathogen to which it has high affinity, it binds to it which can disable it or alert the immune system for its destruction (4).
Plants do not normally produce antibodies and thus must be genetically modified to produce plantibodies as well as foreign protein antigens. Plantibodies produced in this manner function the same way as the antibodies native to the human body (1). The main ways to do this are to stably integrate foreign DNA into a host cell and place it into a plant embryo resulting in a permanent change of the nuclear genome, or to induce transient gene expression of the specified protein (5). In both cases, the genetic material introduced to the plant codes for the protein of choice. Several of the methods used to induce permanent transgene expression include agrobacterium-mediated transformation, particle bombardment using a gene gun, or the transformation of organelles such as chloroplasts. Transient transgene expression can be done using plant viruses as viral vectorsoragroinfiltration (2). Once the genetic material has been inserted, the specified protein is produced via the plant endomembrane and secretory systems, after which it can be recovered through purification of the plant tissue to be used for injection (1). The production of these proteins can also be directed to specific organs of the plant such as the seeds using targeting signals (2). Stable integration techniques are generally used for more large scale production and when the gene in question has a high level of expression, while transient techniques are used to produce a greater yield in the short term (5).
Now how can plantibodies and plant produced antigens help us as humans? The primary purpose of producing plantibodies is for the treatment of disease via immunotherapy. Immunotherapy is a method of treatment in which one’s immune response to a particular disease is enhanced. Specific plantibodies can be produced in order to target a particular disease and then be applied to patients via injection as a means of treatment (6). Doing so provides a boost to the number of antibodies against the targeted disease in the patient’s body which helps to enhance their immune system response against it. An example of this is CaroRx, the first clinically tested plantibody which has the ability to bind to Streptococcus mutans. CaroRx has been shown to be effective in the treatment of tooth decay caused by this species of bacteria (1). More recently, a plantibody known as ZMapp has shown potential in the treatment of Ebola. A study by Qiu et al showed that when administered up to 5 days after the onset of the disease, 100% of rhesus macaques that were administered the drug were shown to have recovered from its effects while all of the control group animals perished as a result of the disease (7). In addition, it has been experimentally administered to some humans who later recovered from the disease, although its role in their recovery was not fully ascertained (8).
Plant produced antigens on the other hand can be used to produce oral vaccines (9). Vaccines are typically biological mixtures containing a weakened pathogen and its antigens. Injection of this results in priming of the body’s adaptive immune system against the particular pathogen so that it can more easily recognize and respond to the threat in the future (4). By producing the antigens of targeted pathogens in plants through transgenic expression, edible vaccines can be created if the plant used is safe to eat. Tobacco, potato, and tomato plants have typically been used in past attempts to create them, showing success in both animal studies and a number of human trials. The advantages of using an oral vaccine include ease of administration and lower costs since specialised personel are not required for administration (9). In addition, oral vaccines are more effective in providing immunity against pathogens at mucosal surfaces as they can be directly applied to the gastrointestinal tract (1). The primary issue with the usage of oral vaccines is that protein antigens must avoid degradation in the stomach and intestines before they can reach the targeted sites in the body. Several solutions to this dilemma include using other biological structures such as liposomesandproteasomes as a means of delivery. This helps to prevent the proteins from being degraded by digestive enzymes and the acidic environment of the stomach before they can reach their destination (1, 9).
There are a number of advantages to using these plant based pharmaceuticals. First of all, they can be produced on a large scale at a relatively low cost through agriculture and are convenient for long-term storage due to the resiliency and size of plant seeds (5). There is also a low risk of contamination by mammalian viruses, blood borne pathogens, and oncogenes which can remove the need for expensive removal steps (1). In addition, purification steps can be skipped if the plants used are edible and ethical problems that come with animal production can be avoided (5). The disadvantages include the potential for allergic reactions to plant antigens and contamination by pesticides and herbicides. There is also the possibility of outcrossing of transgenic pollen to weeds or related crops which would lead to non-target crops also expressing the pharmaceutical.This could lead to public concern along with the potential that other species which ingest these plants may be negatively affected (9). While plantibodies and plant produced antigens have not yet been extensively tested in clinical trials, going forward they represent a new treatment option with great promise.
References
1. Jain P, Pandey P, Jain D, Dwivedi P. Plantibody: An overview. Asian journal of Pharmacy and Life Science. 2011 Jan;1(1):87-94.
2. Stoger E, Sack M, Fischer R, Christou P. Plantibodies: applications, advantages and bottlenecks. Current Opinion in Biotechnology. 2002 Apr 1;13(2):161-166.
3. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular Biology of the Cell. 4th Edition. New York: Garland Science; 2002.
4. Parham P. The immune system. 4th Edition. New York: Garland Science; 2014.
5. Ferrante E, Simpson D. A review of the progression of transgenic plants used to produce plantibodies for human usage. J. Young Invest. 2001;4:1-0.
6. Smith MD. Antibody production in plants. Biotechnology advances. 1996 Dec 31;14(3):267-81.
7. Qiu X, Wong G, Audet J, Bello A, Fernando L, Alimonti JB, Fausther-Bovendo H, Wei H, Aviles J, Hiatt E, Johnson A. Reversion of advanced Ebola virus disease in nonhuman primates with ZMapp. Nature. 2014 Aug 29.
8. Sneed A. Know the Jargon. Scientific american. 2014 Dec 1;311(6):24-24.
9. Daniell H, Streatfield SJ, Wycoff K. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends in plant science. 2001 May 1;6(5):219-26.
Much of my research deals with the ways in which bodies are disciplined and how they go about resisting that discipline. In this piece, adapted from one of the answers to my PhD preliminary exams written and defended two months ago, I “name the disciplinary strategies that are used to control bodies and discuss the ways that bodies resist those strategies.” Additionally, I address how strategies of embodied control and resistance have changed over time, and how identifying and existing as a cyborg and/or an artificial intelligence can be understood as a strategy of control, resistance, or both.
In Jan Golinski’s Making Natural Knowledge, he spends some time discussing the different understandings of the word “discipline” and the role their transformations have played in the definition and transmission of knowledge as both artifacts and culture. In particular, he uses the space in section three of chapter two to discuss the role Foucault has played in historical understandings of knowledge, categorization, and disciplinarity. Using Foucault’s work in Discipline and Punish, we can draw an explicit connection between the various meanings “discipline” and ways that bodies are individually, culturally, and socially conditioned to fit particular modes of behavior, and the specific ways marginalized peoples are disciplined, relating to their various embodiments.
This will demonstrate how modes of observation and surveillance lead to certain types of embodiments being deemed “illegal” or otherwise unacceptable and thus further believed to be in need of methodologies of entrainment, correction, or reform in the form of psychological and physical torture, carceral punishment, and other means of institutionalization.
✨Almost Real: A Speculative Biology Zine (Vol. 4 - BIOTECHNOLOGY) launches March 20th on Kickstarter! ✨
Pull up a chair, dust off those blueprints, and see what specimens you’re working with as Almost Real’s 4th Volume takes you on a journey through biotech of all shapes and sizes.
i’m not sure if you’ve come across this or not, but the artist eduardo kac created a piece called ‘natural history of the enigma’ in wich he genetically engineered the flower to become a hybird of himself and his own dna - the red veins in the petals hold his dna. really interesting :) see eduardokac.org
Thanks! That doesn’t appear to be the correct website though. Readers, go here if you’d like to learn more about this:
